ELISpot Flex: Rabbit IgG (HRP)

ELISpot Flex: Rabbit IgG (HRP)

3152-2H

Publications: 1
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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAbMT5131
Detection mAbMT536, biotin
Enzyme conjugateStreptavidin-HRP

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Delivery 4-9 business days

Shipping $0

Complementary products

Complementary products
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ELISpot substrate: TMB for HRP


Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of B cells secreting rabbit IgG using the ELISpot assay. B cells secreting antigen-specific IgG, as well as B cells secreting IgG irrespective of antigen specificity (total IgG) can be enumerated. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

The assay is recommended for studies of in vivo activated B cells, e.g. after immunization. The time-point for detection of cells secreting antigen-specific IgG after immunization needs to be established by the user.

Antigen-specific B cells can be enumerated in two alternative ways; by coating with antigen or by using biotinylated antigen in the detection step.

Recommendation

Product details

ProductELISpot Flex: Rabbit IgG (HRP)
ApplicationELISpot
AnalyteIgG
ReactivityRabbit
Specificity

This kit is based on a matched pair of mAbs specific for rabbit IgG.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store at 4-8 °C upon receipt.

Shelf lifeAt least 18 months from date of receipt.
Spleen cells from OVA immunized rabbit (150,000 cells/well) incubated overnight in the ELISpot plate. Spots were detected with biotinylated antigen (OVA-biotin).
Datasheet/Protocol
SDS

Tutorial, Published December 28, 2023

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Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot
In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 21, 2022

B cell profiling with ELISpot and FluoroSpot
ELISpot and FluoroSpot are considered the gold standard method for finding rare, antigen-specific B cells.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them
ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?
DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.
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IgG

Analyte description

Immunoglobulin G (IgG) is the most abundant Ig isotype in serum, making up approximately 80% of all serum immunoglobulins. In humans, there are four subclasses of immunoglobulin G, with the highest serum concentrations of IgG1 followed by IgG2, IgG3, and IgG4. In mice, the IgG subclasses are defined as IgG1, IgG2a/c, IgG2b, and IgG3. The IgG molecule consists of two heavy and two light chains (κ or λ), resulting in a molecule with two arms for antigen binding. High levels of IgG antibodies are induced following the initial IgM response in a typical immune response to antigens.

Alternative namesImmunoglobulin G, IgG
Cell typeB cell